What is DFAST?
DFAST is an automatic annotation service for prokaryotic genomes. It also assists data submission to DDBJ.
Who can use DFAST?
DFAST is freely available for both academic and for-profit users. No user registration is required.
How can I use DFAST?
Just upload your genome sequence data in a FASTA format. Either single- or multi-FASTA file is OK.
DFAST generates annotation results in standard annotation formats such as FASTA, GenBank, and GFF as well as in a DDBJ data submission format.
If I use DFAST, is the result automatically going to be submitted to DDBJ or is the data submission to DDBJ mandatory?
No. To submit your data to DDBJ, you need to send files generated by DFAST by yourself. You can also use DFAST only to annotate your genome without submitting the result to DDBJ.
How is the data confidentiality protected?
A 128-bit unique ID (UUID) will be assigned to the submitted job. Only users who know the ID can access the result.
What kinds of personal information are collected by DFAST?
For security purposes, job submitters' IP addresses are recorded. If provided, job titles and email addresses are collected. We use Google Analytics to survey system usage. Each access information will not be disclosed, but statistics such as annual numbers of processed jobs or visitors might be disclosed.
How long are the results kept?
All the data will be deleted after 30 days have passed since your last access to the result. You can also delete the data immediately by pressing the 'Delete' button in the result page.
I got an error.
Please check the query file. In particular, ambiguous nucleotides such as R (=A or G) or Y (=T or C) are not allowed, which should be replaced with 'N'. Use this script to replace them replace_ambiguous.py.
How can I cite DFAST?
See this page.
Inquiry and feedback.
If you have any question, request, or suggestion, please contact us at dfast[at]nig.ac.jp . Make sure to let us know the job ID for the inquiry regarding job results and errors.

投入されたジョブには128bitのユニークID (UUID) からなるジョブIDが発行され、ジョブIDを知る者のみが結果にアクセスできます。 一般に、UUIDを推測することは実質的に不可能であるとされています。
セキュリティ上の理由からジョブ投入者のアクセス元IPアドレスを収集しています。ユーザーが入力した場合には、ジョブタイトルおよびemailアドレスも記録されます。また、サービスの利用状況を調査するためGoogle Analyticsを使用しています。個別のアクセス情報が開示されることはありませんが、成果報告等において統計値 (年間ジョブ投入数やアクセス数等) を公開することがあります。
アップロードしたファイルをチェックしてください。特に、ambiguous nucleotide [R (=A or G), Y (=T or C) など] が含まれているとエラーが生じますので、これらは不明塩基 N に置換してください。(こちらの変換用Pythonスクリプトをご利用ください replace_ambiguous.py) 原因が不明な場合にはお気軽にお問い合わせください。
dfast[at]nig.ac.jp に連絡をしてください。ジョブ結果についてのお問い合わせは、結果ページのURLをお知らせください。