As of August 2017, we have replaced the background annotation engine of the DFAST web service with our original pipeline called DFAST-core.
DFAST-core is also available as a stand-alone command line program, providing a local annotation platform for sensitive or mass sequence data.


For more information, please visit our GitHub repository.

Overview

Easy install

Runs on Mac and Linux with Python 2.7 and 3.4-.
External binaries are bundled in the software distribution.

Flexible and customizable

You can customize the pipeline by defining your own configuration file.

Fast and rich annotation

Less than 10 minutes for typical-sized bacterial genome annotation.
Unique functions such as orthologous gene assignment between reference genomes, pseudo/frameshifted gene prediction.

INSDC submission

Supports fully qualified DDBJ submission files as well as .tbl and .fsa files for GenBank submission.

Download

Get the latest code at GitHub

DFC source code

Download the DFAST-core distribution

DFC GitHub releases

Reference databases

DFAST default reference database (latest=v1.1.4)
DFAST default reference database (v1.1.4, 20180612, minor fix for protein names)
DFAST default reference database (v1.1.3, 20180516, minor fix for protein names)
DFAST default reference database (v1.1.2, 20180426, minor fix for protein names)
DFAST default reference database (v1.1.1, 20171117)

Lactic acid bacteria (mainly Lactobacillaceae) (1.0)
Escherichia coli (0.1)
Cyanobacteria (0.1)
Bifidobacterium (0.1)
You do not need to download these files manually. Use file_downloader.py script bundled in the software distribution.